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Culture media are artificially formulated nutrients for the growth and maintenance of microorganisms, plant, and animal tissues. The culture medium can be divided into many types according to different classifications, for example, according to physical classification, liquid culture medium, solid medium, semi-solid medium, dehydrated medium, etc.
The steps for aliquoting the medium are as follows:
1.Divide the culture medium into Erlenmeyer flasks and test tubes of different capacities as needed. The amount of sub-packaging should not exceed 2/3 of the container to avoid overflow during sterilization.
2.The volume of the agar slant is 1/5 of the capacity of the test tube. After sterilization, it must be placed on a slanted surface while hot, and the length of the slanted surface is about 2/3 of the length of the test tube.
3.The volume of the semi-solid medium is about 1/3 of the length of the test tube. After sterilization, it will stand upright and solidify for use
4.The volume of high-layer agar is about 1/3 of the test tube. After sterilization, stand upright while it is still hot, and then solidify after cooling.
5.The liquid medium is divided into test tubes, about 1/3 of the length of the test tube.
6.Agar plate: cool the sterilized (or heated and melted) medium to about 50°C, and pour it into a sterilized plate with aseptic procedures. Pour about 13-15ml of the medium into a plate with an inner diameter of 9cm, and shake the bottom of the plate gently. , so that the medium is spread on the bottom of the dish, and it is ready after solidification. When pouring the medium, do not open the lid of the dish completely to prevent dust and bacteria from falling into the air.
The newly made plate medium (referred to as plate) has a lot of water on the surface, which is not conducive to the separation of bacteria. Usually, the plate should be placed upside down in a 37°C incubator for about 30 minutes until the plate surface is dry before use. Compared with humans, the peristaltic pump is more accurate for dispensing. Only need to set the value, you can directly dispense, avoid repetitive and meaningless operations, and focus on other more important things such as research.
Not only the media dispensing, but other fluid dispensing in the laboratory can also use the peristaltic pump for fluid dispensing. Why?
1.When the peristaltic pump is running, the fluid only contacts the pump tube, so there is no need to worry about the liquid being contaminated. For this reason, the wear parts of peristaltic pumps are basically only hoses, and maintenance and replacement are also very simple.
2.Generally, the more intelligent peristaltic pumps can store the transmitted flow, which can realize fast filling without the need to manually set the data.
3.Compared with manual packaging, the peristaltic pump has higher packaging accuracy and is more suitable for use in the environment of laboratory packaging.
The steps for aliquoting the medium are as follows:
1.Divide the culture medium into Erlenmeyer flasks and test tubes of different capacities as needed. The amount of sub-packaging should not exceed 2/3 of the container to avoid overflow during sterilization.
2.The volume of the agar slant is 1/5 of the capacity of the test tube. After sterilization, it must be placed on a slanted surface while hot, and the length of the slanted surface is about 2/3 of the length of the test tube.
3.The volume of the semi-solid medium is about 1/3 of the length of the test tube. After sterilization, it will stand upright and solidify for use
4.The volume of high-layer agar is about 1/3 of the test tube. After sterilization, stand upright while it is still hot, and then solidify after cooling.
5.The liquid medium is divided into test tubes, about 1/3 of the length of the test tube.
6.Agar plate: cool the sterilized (or heated and melted) medium to about 50°C, and pour it into a sterilized plate with aseptic procedures. Pour about 13-15ml of the medium into a plate with an inner diameter of 9cm, and shake the bottom of the plate gently. , so that the medium is spread on the bottom of the dish, and it is ready after solidification. When pouring the medium, do not open the lid of the dish completely to prevent dust and bacteria from falling into the air.
The newly made plate medium (referred to as plate) has a lot of water on the surface, which is not conducive to the separation of bacteria. Usually, the plate should be placed upside down in a 37°C incubator for about 30 minutes until the plate surface is dry before use. Compared with humans, the peristaltic pump is more accurate for dispensing. Only need to set the value, you can directly dispense, avoid repetitive and meaningless operations, and focus on other more important things such as research.
Not only the media dispensing, but other fluid dispensing in the laboratory can also use the peristaltic pump for fluid dispensing. Why?
1.When the peristaltic pump is running, the fluid only contacts the pump tube, so there is no need to worry about the liquid being contaminated. For this reason, the wear parts of peristaltic pumps are basically only hoses, and maintenance and replacement are also very simple.
2.Generally, the more intelligent peristaltic pumps can store the transmitted flow, which can realize fast filling without the need to manually set the data.
3.Compared with manual packaging, the peristaltic pump has higher packaging accuracy and is more suitable for use in the environment of laboratory packaging.
