Application of peristaltic pump in chromatography

04/03/2022
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Chromatography is a kind of mixture separation technology, which is widely used in analytical chemistry, organic chemistry, biochemistry and other fields.Using the difference of physical and chemical properties of each component in the mixture, the multi-component mixture was separated and determined by chromatography.It can be divided into adsorption chromatography, distribution chromatography, ion exchange chromatography, gel filtration chromatography, etc.
Pumping and distributing media, buffer solutions, or other liquids is a common task performed in laboratories.Ensuring that liquids are transferred with high precision and remain free of contamination is a key challenge in using pumps to treat liquids.
Previously, we looked at the peristaltic pump in tissue irrigation, and today we'll look in more detail at how the peristaltic pump contributes to the efficient completion of chromatography experiments.
Chromatography is a kind of mixture separation technology, which is widely used in analytical chemistry, organic chemistry, biochemistry and other fields.Using the difference of physical and chemical properties of each component in the mixture, the multi-component mixture was separated and determined by chromatography.It can be divided into adsorption chromatography, distribution chromatography, ion exchange chromatography, gel filtration chromatography, etc.
Specific applications of chromatography include:
Desalination, such as gel chromatography to remove low molecular weight impurities from solution of polymer (such as protein, nucleic acid, polysaccharide, etc.);
Separation and purification, used for the separation and purification of enzymes, proteins, polysaccharides, hormones, alkaloids and other substances;
Concentration of a dilute solution;
Determination of molecular weight of polymer;
As well as various chromatography method and the packing performance research and so on;
In the natural state, due to the limited penetration rate of liquid in the chromatography column, appropriate external pressure is needed to enhance the penetration capacity of liquid, and ensure that the liquid is free from cross contamination.Therefore, peristaltic pump is an ideal choice for conveying fluid in the process of chromatography, and is often used to control the flow rate of mobile phase, eluent flow rate, pump buffer, and extract samples to be tested.